Polymyxins and analogues bind to ribosomal RNA and interfere with eukaryotic translation in vitro

Abstract

Looking for targets: while the bactericidal activity of polymyxins is attributed to changes in membrane permeation, we show that these antibiotics can bind prokaryotic and eukaryotic A-sites, domains responsible for translational decoding. Polymyxin B, colistin and analogues also hinder eukaryotic translation in vitro. These new targets and effects might be partially responsible for the plethora of adverse effects by these potent bactericidal agents.

Keywords: RNA; antibiotics; colistin; polymyxins; translation.