A novel highly reactive Fab antibody for breast cancer tissue diagnostics and staging also discriminates a subset of good prognostic triple-negative breast cancers

Cancer Lett. 2014 Feb 28;343(2):275-85. doi: 10.1016/j.canlet.2013.09.029. Epub 2013 Oct 4.

Abstract

The discovery of novel markers for breast cancer (BC) has been recently relied on antibody combinatorial libraries and selection through phage display. We constructed a recombinant Fab library, and after selections against BC tissues, the FabC4 clone was thoroughly investigated by immunohistochemistry in 232 patients with long-term follow-up. The FabC4 ligand was determined by mass spectrometry. The FabC4 expression was associated with younger age, lack of progesterone receptor, higher histological grades and non-luminal subtypes, and it also identified a subset of good prognostic triple-negative BCs, possibly targeting a conformational epitope of Cytokeratin-10 (CK10). This new CK10-epitope specific antibody may open new possibilities in diagnostic and therapeutic strategies.

Keywords: Breast cancer; Combinatorial library; Phage display; Recombinant antibody.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Amino Acid Sequence
  • Breast Neoplasms / diagnosis*
  • Disease-Free Survival
  • Female
  • Humans
  • Immunoglobulin Fab Fragments* / genetics
  • Mass Spectrometry
  • Microarray Analysis
  • Middle Aged
  • Neoplasm Staging
  • Peptide Library
  • Prognosis
  • Triple Negative Breast Neoplasms / diagnosis*

Substances

  • Immunoglobulin Fab Fragments
  • Peptide Library