Abstract
A convenient scaffold based on poly(N-vinyl-2-pyrrolidone-co-vinyl alcohol) is proposed for presenting ligands in multivalent format. This amphiphilic polymer supports synthesis of conjugates in both organic and aqueous media, permits enzymatic processing of the ligand precursor, and, finally, offers a choice of formats for evaluation of biological activity either as a soluble inhibitor or as a capture reagent after deposition on a hydrophobic surface or standard microtiter plates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites / drug effects
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Dose-Response Relationship, Drug
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Enzyme-Linked Immunosorbent Assay
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Ligands
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Molecular Probes / chemical synthesis
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Molecular Probes / chemistry*
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Molecular Probes / pharmacology
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Molecular Structure
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Polyvinyl Alcohol / analogs & derivatives*
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Polyvinyl Alcohol / chemical synthesis
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Polyvinyl Alcohol / chemistry
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Povidone / analogs & derivatives*
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Povidone / chemical synthesis
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Povidone / chemistry
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Shiga Toxin 1 / antagonists & inhibitors
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Shiga Toxin 1 / metabolism
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Structure-Activity Relationship
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Surface-Active Agents / chemical synthesis
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Surface-Active Agents / chemistry*
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Surface-Active Agents / pharmacology
Substances
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Ligands
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Molecular Probes
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Shiga Toxin 1
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Surface-Active Agents
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poly(vinyl alcohol-co-N-vinyl-2-pyrrolidone)
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Polyvinyl Alcohol
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Povidone