Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori

PLoS One. 2013 Sep 30;8(9):e75731. doi: 10.1371/journal.pone.0075731. eCollection 2013.

Abstract

Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, and as well as by Trans Proteomic Pipeline (TPP) and Absolute protein expression (APEX) analyses. Forty-one proteins were further identified by two-dimensional gel electrophoresis. Some proteins were revealed to be significantly associated with various metabolic processes, organic solute transport, detoxification and innate immunity. Our results might lay a good foundation for future functional studies of MTs in silkworm and other lepidoptera.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Bombyx / genetics*
  • Bombyx / metabolism
  • Chromatography, Liquid
  • Computational Biology
  • DNA Primers / genetics
  • Electrophoresis, Gel, Two-Dimensional
  • Malpighian Tubules / metabolism*
  • Proteome / genetics*
  • Proteome / metabolism
  • Proteomics
  • Tandem Mass Spectrometry

Substances

  • DNA Primers
  • Proteome

Grants and funding

This work was supported by grants from the National Basic Research Program of China (Grant No. 2012CB114604), http://www.973.gov.cn/Default_3.aspx, the National Hi-Tech Research and Development Program of China (Grant No. 2011AA100306), http://www.863.gov.cn/, the National Natural Science Foundation (Grant No. 31172157), http://www.nsfc.gov.cn/Portal0/default152.htm. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.