DNA adsorption characteristics of hollow spherule allophane nano-particles

Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):5079-83. doi: 10.1016/j.msec.2013.08.040. Epub 2013 Sep 7.

Abstract

To understand the propensity of natural allophane to adsorb the DNA molecules, the adsorption characteristics were assessed against natural allophane (AK70), using single-stranded DNA (ss-DNA) and adenosine 5'-monophosphate (5'-AMP) as a reference molecule. The adsorption capacity of ss-DNA on AK70 exhibited one order of magnitude lower value as compared with that of 5'-AMP. The adsorption capacity of ss-DNA decreased with increasing pH due to the interaction generated between phosphate groups of ss-DNA and functional Al-OH groups on the wall perforations through deprotonating, associated with higher energy barrier for the adsorption of ss-DNA. The adsorption morphologies consisting of the individual ss-DNA with mono-layer coverage of the clustered allophane particle were observed successfully through transmission electron microscopy analysis.

Keywords: Adsorption; Clustered particles; Natural allophane; Single-stranded DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Monophosphate / chemistry
  • Adsorption
  • Aluminum Silicates / chemistry*
  • Clay
  • DNA, Single-Stranded / chemistry*
  • Nanoparticles / chemistry*
  • Surface Properties

Substances

  • Aluminum Silicates
  • DNA, Single-Stranded
  • aluminosilicate
  • Adenosine Monophosphate
  • Clay