Improving the cellular invasion into PHEMA sponges by incorporation of the RGD peptide ligand: the use of copolymerization as a means to functionalize PHEMA sponges

Stefan M Paterson; Audra M A Shadforth; Jeremy A Shaw; David H Brown; Traian V Chirila; Murray V Baker

doi:10.1016/j.msec.2013.08.011

Improving the cellular invasion into PHEMA sponges by incorporation of the RGD peptide ligand: the use of copolymerization as a means to functionalize PHEMA sponges

Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4917-22. doi: 10.1016/j.msec.2013.08.011. Epub 2013 Aug 17.

Authors

Stefan M Paterson¹, Audra M A Shadforth, Jeremy A Shaw, David H Brown, Traian V Chirila, Murray V Baker

Affiliation

¹ School of Chemistry and Biochemistry, The University of Western Australia (M313), Crawley, WA 6009, Australia.

PMID: 24094205
DOI: 10.1016/j.msec.2013.08.011

Abstract

A monomer that contained the RGD ligand motif was synthesized and copolymerized with 2-hydroxyethyl methacrylate using polymerization-induced phase separation methods to form poly(2-hydroxyethyl methacrylate)-based hydrogel sponges. The sponges had morphologies of aggregated polymer droplets and interconnected pores, the pores having dimensions in the order of 10 μm typical of PHEMA sponges. RGD-containing moieties appeared to be evenly distributed through the polymer droplets. Compared to PHEMA sponges that were not functionalized with RGD, the new sponges containing RGD allowed greater invasion by human corneal epithelial cells, by advancing the attachment of cells to the surface of the polymer droplets.

Keywords: Cell invasion; PHEMA; Peptide ligands; Sponges.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocompatible Materials / chemistry*
Biocompatible Materials / pharmacology
Cell Adhesion / drug effects
Cells, Cultured
Epithelial Cells / cytology
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology
Ligands
Microscopy, Electron, Transmission
Oligopeptides / chemistry*
Polyhydroxyethyl Methacrylate / chemistry*

Substances

Biocompatible Materials
Ligands
Oligopeptides
Polyhydroxyethyl Methacrylate
Hydrogel, Polyethylene Glycol Dimethacrylate
arginyl-glycyl-aspartic acid