Effect of Bothrops bilineata snake venom on neutrophil function

Toxicon. 2013 Dec 15:76:143-9. doi: 10.1016/j.toxicon.2013.09.019. Epub 2013 Sep 27.

Abstract

The aim of the study was to evaluate the in vitro effects of Bothrops bilineata crude venom (BbV) on isolated human neutrophil function. We proved that BbV isn't toxic towards human neutrophils. During an incubation of human neutrophils with BbV hydrogen peroxide was produced. Moreover, BbV was able to stimulate neutrophil release of proinflammatory mediators such as IL-8 and IL-6 as well as PGE2 and NETs liberation. There is no data in the literature showing the effect of BbV on the production of IL-6 and IL-8 or NETs liberation by isolated human neutrophils. Taken together our results testify that BbV triggers relevant proinflammatory events in human neutrophils.

Keywords: Bothrops (Bothriopsis) bilineata venom; Cytokines; NETs; Neutrophils; Reactive oxygen species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bothrops*
  • Crotalid Venoms / toxicity*
  • Dinoprostone / metabolism
  • Humans
  • Hydrogen Peroxide / metabolism
  • Interleukin-6 / metabolism
  • Interleukin-8 / metabolism
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • Neutrophils / physiology
  • Toxicity Tests

Substances

  • Crotalid Venoms
  • Interleukin-6
  • Interleukin-8
  • Hydrogen Peroxide
  • Dinoprostone