Interaction peculiarities of 5,10,15,20-tetrakis(4-N-methylpyridil) tetra iodide porphyrin with albumin

Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jan 24:118:395-8. doi: 10.1016/j.saa.2013.06.101. Epub 2013 Jul 16.

Abstract

In present work interactions of bovine serum albumin with 5,10,15,20-tetrakis(4-N-methylpyridil) tetra iodide porphyrin have been studied by electron absorption and fluorescence spectroscopy. The studies were carried out in aqueous media at different pH and in water-dimethylformamide mixtures containing up to 0.19 M of the organic solvent. It has been demonstrated that the porphyrin forms stable complexes with BSA in which the porphyrin is located subdomains IB and IIA. The stability constants of the complexes is practically independent of pH.

Keywords: Bovine serum albumin; Electronic absorption; Fluorescence spectra; Polymer complex; Porphyrins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Iodides / chemistry
  • Iodides / metabolism*
  • Models, Molecular
  • Porphyrins / chemistry
  • Porphyrins / metabolism*
  • Protein Structure, Tertiary
  • Serum Albumin, Bovine / chemistry
  • Serum Albumin, Bovine / metabolism*
  • Spectrometry, Fluorescence

Substances

  • Iodides
  • Porphyrins
  • Serum Albumin, Bovine