TCTP is a critical factor in shrimp immune response to virus infection

PLoS One. 2013 Sep 20;8(9):e74460. doi: 10.1371/journal.pone.0074460. eCollection 2013.

Abstract

The translationally controlled tumor protein (TCTP) is an abundant, ubiquitous, and conserved protein which plays important roles in a number of biological processes. In the present study, the TCTP in shrimp Litopenaeus vannamei was analyzed. The TCTP of L.vannamei, a 168-amino-acid polypeptide, shares a high degree of similarity with TCTPs from other species, having two TCTP protein signatures at the 45-55 aa and 123-145 aa motif. The mRNA and protein levels from different tissues were detected with the highest in muscle and the lowest in heart among all examined tissues. In addition, temporal TCTP expression was significantly up-regulated at 16 h and 48 h following infection with white spot syndrome virus (WSSV). Lastly, silencing of TCTP with dsRNA led to a significant increase of WSSV loads. These results provide new insights into the importance of TCTP as an evolutionarily conserved molecule for shrimp innate immunity against virus infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / immunology
  • Biomarkers, Tumor / metabolism*
  • Blotting, Western
  • Gene Expression Regulation
  • Immunity, Innate / immunology*
  • Molecular Sequence Data
  • Penaeidae / genetics
  • Penaeidae / immunology*
  • Penaeidae / virology*
  • Phylogeny
  • RNA, Double-Stranded / genetics
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tumor Protein, Translationally-Controlled 1
  • White spot syndrome virus 1 / pathogenicity*

Substances

  • Biomarkers, Tumor
  • RNA, Double-Stranded
  • RNA, Messenger
  • Tumor Protein, Translationally-Controlled 1

Grants and funding

This research was supported by the National Natural Science Foundation of China (31072236), the Education Office of Fujian Province (JK2009043) and Haixi Project of Fujian (A101). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.