Gp10 based-thioetherification (10BASE(d)-T) on a displaying library peptide of bacteriophage T7

Keisuke Fukunaga; Takaaki Hatanaka; Yuji Ito; Masumi Taki

doi:10.1039/c3mb70379g

Gp10 based-thioetherification (10BASE(d)-T) on a displaying library peptide of bacteriophage T7

Mol Biosyst. 2013 Dec;9(12):2988-91. doi: 10.1039/c3mb70379g. Epub 2013 Sep 27.

Authors

Keisuke Fukunaga¹, Takaaki Hatanaka, Yuji Ito, Masumi Taki

Affiliation

¹ Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC), 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan. taki@pc.uec.ac.jp.

PMID: 24072138
DOI: 10.1039/c3mb70379g

Abstract

The site-specific introduction of a haloacetamide derivative into a designated cysteine on a displaying peptide on a capsid protein (gp10) of bacteriophage T7 has been achieved. This easiest gp10-based thioetherification (10BASEd-T) is carried out in one-pot without side reactions or loss of phage infectivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacteriophage T7 / metabolism*
Capsid Proteins / chemistry*
Capsid Proteins / metabolism
Peptide Library
Protein Processing, Post-Translational
Rhodamines / chemistry
Sulfides / chemistry*

Substances

Capsid Proteins
Peptide Library
Rhodamines
Sulfides
tetramethylrhodamine