Abstract
Three structural domains of mosquitocidal Cry11Aa and Cry11Ba from Bacillus thuringiensis were exchanged to produce interdomain chimeras [BAA (11Ba/11Aa/11Aa), ABA (11Aa/11Ba/11Aa), AAB (11Aa/11Aa/11Ba), ABB (11Aa/11Ba/11Ba), BAB (11Ba/11Aa/11Ba), BBA (11Ba/11Ba/11Aa]. Chimeras BAB, BAA, BBA, and AAB formed inclusion bodies in the crystal-negative B. thuringiensis host and produced expected protein bands on SDS-PAGE gel. However, no inclusion body or target protein could be found for chimeras ABA and ABB. In bioassays using the fourth-instar larvae of Culex quinquefasciatus and Aedes aegypti, AAB had ~50 % lethal concentrations of 4.8 and 2.2 μg ml(-1), respectively; however, the rest of chimeras were not toxic. This study thus helps to understand the domain-function relationships of the Cry11Aa and Cry11Ba toxins. The toxic chimera, AAB, might be a candidate for mosquito control as its amino acid sequence is different from the two parental toxins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacillus thuringiensis Toxins
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Bacterial Proteins / pharmacology
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Bacterial Toxins / chemistry*
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Bacterial Toxins / genetics*
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Bacterial Toxins / metabolism
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Bacterial Toxins / pharmacology
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Culex / drug effects
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Electrophoresis, Polyacrylamide Gel
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Endotoxins / chemistry*
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Endotoxins / genetics*
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Endotoxins / metabolism
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Endotoxins / pharmacology
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Escherichia coli
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Hemolysin Proteins / chemistry*
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Hemolysin Proteins / genetics*
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Hemolysin Proteins / metabolism
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Hemolysin Proteins / pharmacology
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Insecticides / chemistry*
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Insecticides / metabolism
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry*
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Recombinant Fusion Proteins / genetics*
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / pharmacology
Substances
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Bacterial Toxins
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CRY11BB protein, Bacillus thuringiensis
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Endotoxins
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Hemolysin Proteins
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Insecticides
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Recombinant Fusion Proteins
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insecticidal crystal protein, Bacillus Thuringiensis