Abstract
Restriction fragment length polymorphisms of the heat-shock protein 70 gene have been used for discriminating Leishmania species. Here, we validated HindII as a much cheaper alternative to EcoRII and SduI for discriminating Leishmania (Viannia) braziliensis from Leishmania (Viannia) naiffi and an atypical Leishmania (V.) braziliensis group, which was previously not possible.
Keywords:
Leishmania; PCR-RFLP; Typing; hsp70.
© 2013.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Protozoan / genetics
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DNA, Protozoan / metabolism
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Deoxyribonucleases, Type II Site-Specific / metabolism
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HSP70 Heat-Shock Proteins / genetics*
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Leishmania / classification*
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Leishmania / genetics*
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Molecular Typing / methods*
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Parasitology / methods*
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length*
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Protozoan Proteins / genetics*
Substances
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DNA, Protozoan
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HSP70 Heat-Shock Proteins
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Protozoan Proteins
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Deoxyribonucleases, Type II Site-Specific
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GDGCHC-specific GATC-specific type II deoxyribonucleases
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GTYRAC-specific type II deoxyribonucleases