HindII and SduI digests of heat-shock protein 70 PCR for Leishmania typing

Diagn Microbiol Infect Dis. 2013 Nov;77(3):245-7. doi: 10.1016/j.diagmicrobio.2013.07.023. Epub 2013 Sep 17.

Abstract

Restriction fragment length polymorphisms of the heat-shock protein 70 gene have been used for discriminating Leishmania species. Here, we validated HindII as a much cheaper alternative to EcoRII and SduI for discriminating Leishmania (Viannia) braziliensis from Leishmania (Viannia) naiffi and an atypical Leishmania (V.) braziliensis group, which was previously not possible.

Keywords: Leishmania; PCR-RFLP; Typing; hsp70.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Protozoan / genetics
  • DNA, Protozoan / metabolism
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • HSP70 Heat-Shock Proteins / genetics*
  • Leishmania / classification*
  • Leishmania / genetics*
  • Molecular Typing / methods*
  • Parasitology / methods*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • Protozoan Proteins / genetics*

Substances

  • DNA, Protozoan
  • HSP70 Heat-Shock Proteins
  • Protozoan Proteins
  • Deoxyribonucleases, Type II Site-Specific
  • GDGCHC-specific GATC-specific type II deoxyribonucleases
  • GTYRAC-specific type II deoxyribonucleases