Abstract
A fibrin hydrogel at physiological concentration (5 mg/mL) was associated with polyvinyl alcohol (PVA) inside an interpenetrating polymer networks (IPN) architecture. Previously, PVA has been modified with methacrylate functions in order to cross-link it by free-radical polymerization. The fibrin network was synthesized by the enzymatic hydrolysis of fibrinogen by thrombin. The resulting self-supported materials simultaneously exhibit the properties of the fibrin hydrogel and those of the synthetic polymer network. Their storage modulus is 50-fold higher than that of the fibrin hydrogel and they are completely rehydratable. These materials are noncytotoxic toward human fibroblast and the fibrin present on the surface of PVAm-based IPNs favors cell development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocompatible Materials / chemistry*
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Biocompatible Materials / metabolism
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Biocompatible Materials / pharmacology
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Cell Survival / drug effects
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Cells, Cultured
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Dose-Response Relationship, Drug
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Fibrin / chemistry*
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Fibrin / metabolism
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Fibrinogen / chemistry
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Fibrinogen / metabolism
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Foreskin / cytology
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Foreskin / drug effects
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Foreskin / metabolism
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Humans
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Hydrolysis
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Male
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Particle Size
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Polyvinyl Alcohol / chemistry*
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Polyvinyl Alcohol / metabolism
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Polyvinyl Alcohol / pharmacology
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Structure-Activity Relationship
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Surface Properties
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Thrombin / metabolism
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Water / chemistry
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Water / metabolism
Substances
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Biocompatible Materials
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Water
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Fibrin
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Fibrinogen
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Polyvinyl Alcohol
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Thrombin