Cell chip with a thiolated chitosan self-assembled monolayer to detect the effects of anticancer drugs on breast normal and cancer cells

Abstract

Cell-based chips are an effective in vitro analysis tool; however, the sensitivity of the cell chip to biomaterials is high, which is crucial for immobilizing cells on the electrode surface without conductivity. In this study, we report on a cell chip with a thiolated chitosan monolayer that was easy to fabricate, highly adhesive to cells, and enhanced electrochemical signals. Thiolated chitosan containing thiol groups was synthesized and self-assembled on a gold electrode to immobilize cells, and showed superior electrochemical performance to that of poly-l-lysine and collagen. Cyclic voltammetry (CV) was performed to distinguish the redox characteristics of normal (HMEC) and breast cancer cells (MCF-7); then, two anticancer drugs (doxorubicin and cyclophosphamide) were added to the cell cultures to analyze their effects on the redox environment of normal and cancer cells derived from the same origin. As a result, the CV cathode peaks decreased differently with respect to the cell line (normal and cancer) and anticancer drug, which was validated by a conventional MTT viability assay. Hence, the proposed cell chip with a thiolated chitosan modified layer could be used in various fields, including discriminating normal from cancer cells, to evaluating the efficiency of newly developed drugs, and to assessing cytotoxicity of various chemicals.

Keywords: A thiolated chitosan modified chip; Cyclic voltammetry; HMEC; MCF-7; MTT assay.