Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus

Diagn Microbiol Infect Dis. 2013 Nov;77(3):270-2. doi: 10.1016/j.diagmicrobio.2013.06.006. Epub 2013 Sep 10.

Abstract

We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA submitted to a surveillance network.

Keywords: Chlorhexidine; MRSA; MupA; Mupirocin; QacA.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Validation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Chlorhexidine / pharmacology
  • Drug Resistance, Bacterial*
  • Humans
  • Membrane Transport Proteins / genetics*
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Mupirocin / pharmacology
  • Nuclear Proteins / genetics*
  • Polymerase Chain Reaction / methods*
  • Staphylococcal Infections / microbiology*
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • FemA protein, Bacteria
  • Membrane Transport Proteins
  • Nuclear Proteins
  • QacB protein, Staphylococcus aureus
  • mupA protein, Staphylococcus aureus
  • qacA protein, Staphylococcus aureus
  • Mupirocin
  • Chlorhexidine