Structural analysis of RNA backbone using in-line probing

Ali Nahvi; Rachel Green

doi:10.1016/B978-0-12-420037-1.00022-1

Structural analysis of RNA backbone using in-line probing

Methods Enzymol. 2013:530:381-97. doi: 10.1016/B978-0-12-420037-1.00022-1.

Authors

Ali Nahvi¹, Rachel Green

Affiliation

¹ Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

PMID: 24034334
DOI: 10.1016/B978-0-12-420037-1.00022-1

Abstract

To determine the secondary structure characteristics of nucleotides in an RNA polymer.

Keywords: In vitro RNA transcription; In-line probing; Labeling RNA; Polyacrylamide gel electrophoresis (PAGE); Selective 2′-hydroxyl acetylation and primer extension (SHAPE).

MeSH terms

Acetylation
Electrophoresis, Gel, Two-Dimensional / methods
Nucleic Acid Conformation
RNA / chemistry*
RNA / genetics
Transcription, Genetic

Substances

RNA