Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Protein Expr Purif. 2013 Nov;92(1):67-76. doi: 10.1016/j.pep.2013.09.001. Epub 2013 Sep 8.

Abstract

Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.

Keywords: CHO-DG44; HEK-293; Mammalian cells; Orbital shaking; Polyethylenimine; Recombinant protein; Suspension culture; Transfection; Transient gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cell Culture Techniques / instrumentation
  • Cricetinae
  • Cricetulus
  • Drug Carriers / metabolism*
  • Equipment Design
  • Genetic Vectors / administration & dosage*
  • HEK293 Cells
  • Humans
  • Polyethyleneimine / metabolism*
  • Recombinant Proteins / genetics
  • Transfection / methods*
  • Viruses / genetics

Substances

  • Drug Carriers
  • Recombinant Proteins
  • Polyethyleneimine