Abstract
The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca(2+)-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Courtship*
-
Drosophila Proteins / genetics
-
Drosophila Proteins / metabolism
-
Drosophila melanogaster / physiology*
-
Female
-
Ion Channels
-
Male
-
Nerve Tissue Proteins / genetics
-
Neurons / cytology
-
Neurons / metabolism
-
Pheromones / metabolism*
-
Repressor Proteins / genetics
-
Sexual Behavior, Animal / physiology*
-
TRPA1 Cation Channel
-
TRPC Cation Channels / genetics
-
Transcription Factors / genetics
-
Transcription Factors / metabolism
Substances
-
Drosophila Proteins
-
GAL4 protein, Drosophila
-
Ion Channels
-
Nerve Tissue Proteins
-
Pheromones
-
Repressor Proteins
-
TRPA1 Cation Channel
-
TRPC Cation Channels
-
Transcription Factors
-
TrpA1 protein, Drosophila
-
fru protein, Drosophila