Restrictionless cloning

Abstract

Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are contained within the insert to be cloned.

Keywords: Denaturation and annealing; Ligation and transformation; Polymerase chain reaction (PCR); Primer design; Restrictionless cloning; Vector preparation.