Objective: To optimize the extraction conditions of polysaccharide from Moerella iridescens with the microwave-assisted extraction technology by Response surface methodology (RSM).
Methods: Extracting temperature, extracting time and the solvent/solid ratio were selected as factors. The yield of polysaccharide was used as the responsive value. Based on the single factors experiments, a three-factor-three-level experiments design was developed by Box-Benhken central composite design method. The response surface method was employed to analyze the results of experiments.
Results: The optimum extraction conditions of polysaccharide were as follows: extracting temperature was 45 degrees C, extracting time was 2.7 h and solvent/solid ratio was 50:1. The predictive maximum yield of polysaccharide was 1.17%. The average yield of polysaccharide in 3 validation experiments was 1.19% and the relative error was 1.70%.
Conclusion: RSM analysis optimizing the extraction conditions for polysaccharide from Moerella iridescens is feasible, which can improve the extraction rate of Moerella iridescens polysaccharide.