In the course of our ongoing work on the chemical characterization of Taxus baccata L. growing wild in Corsica, we have developed and validated a method for direct quantification of taxane derivatives by (13)C NMR using 10-deacetylbaccatin III as reference compound and 1,6-hexanediol as internal standard. We have observed good accuracy (relative errors between 0.3% and 3.5%), linearity (R(2) = 0.999) and precision (reproducibility 8.5 mg ± 1.1%) of the measurements. The experimental procedure was applied to the quantification of six identified taxanes in a fraction of chromatography of a methanol extract of T. baccata leaves. This method can be applied to other compounds bearing the taxane skeleton.
Keywords: 13C NMR; Taxus baccata L; quantification; taxanes.
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