Methods to quantify microRNAs in the Myc gene network for posttranscriptional gene repression

Methods Mol Biol. 2013:1012:135-44. doi: 10.1007/978-1-62703-429-6_10.

Abstract

As a global transcription factor, Myc regulates both protein-coding genes and noncoding microRNA genes. Myc-activated or repressed miRNAs are involved in various pathways to affect tumorigenesis, mediate apoptosis, proliferation, angiogenesis, metastasis, and metabolism downstream of Myc. Functional characterization of miRNAs in the Myc network requires the accurate detection and quantification of miRNA expression levels. Here, we describe two widely used methodologies to determine miRNA expression, including miRNA real-time PCR and miRNA northern analysis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Blotting, Northern* / methods
  • Gene Expression Regulation, Neoplastic*
  • Gene Regulatory Networks*
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Proto-Oncogene Proteins c-myc / genetics*
  • Proto-Oncogene Proteins c-myc / metabolism
  • RNA Processing, Post-Transcriptional*
  • Real-Time Polymerase Chain Reaction* / methods

Substances

  • MicroRNAs
  • Proto-Oncogene Proteins c-myc