Controlled cell adhesion on poly(dopamine) interfaces photopatterned with non-fouling brushes

Adv Mater. 2013 Nov 13;25(42):6123-7. doi: 10.1002/adma.201302492. Epub 2013 Sep 3.

Abstract

Bioinspired poly(dopamine) (PDA) films are merged with antifouling poly(MeOEGMA) brushes utilizing a nitrile imine-mediated tetrazole-ene cycloaddition (NITEC)-based phototriggered surface encoding protocol. The antifouling brushes were photopatterned on PDA surfaces, leading cells to form confluent layers in the non-irradiated sections, while no adhesion occurred on the brushes resulting in a remarkably precise cell pattern. The presented strategy paves the way for the design of tailor-made patterned cell interfaces.

Keywords: antifouling polymer brushes; micropatterning; nitrile imine-mediated tetrazole-ene cycloaddition (NITEC); photoligation; poly(dopamine).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylates / chemistry
  • Acrylates / metabolism
  • Animals
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism
  • Cell Adhesion*
  • Cell Line
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Indoles / chemistry*
  • Indoles / metabolism
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / metabolism
  • Polymers / chemistry*
  • Polymers / metabolism
  • Rats
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Silicon / chemistry

Substances

  • Acrylates
  • Cell Adhesion Molecules
  • Indoles
  • Microfilament Proteins
  • Phosphoproteins
  • Polymers
  • Recombinant Fusion Proteins
  • poly(oligoethylene glycol methyl ether acrylate)
  • polydopamine
  • vasodilator-stimulated phosphoprotein
  • Green Fluorescent Proteins
  • Polyethylene Glycols
  • Silicon