Abstract
We report the effect of UV-Vis light on the membrane-bound [Ni-Fe] hydrogenase from Aquifex aeolicus under turnover conditions. Using electrochemistry, we show a potential dependent light sensitivity and propose that a light-induced structural change of the [Ni-Fe] active site is related to an enhanced reactivation of the hydrogenase under illumination at high potentials.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / enzymology*
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Bacterial Proteins / chemistry*
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Bacterial Proteins / metabolism
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Enzyme Activation
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Hot Temperature
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Hydrogenase / chemistry*
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Hydrogenase / metabolism*
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Kinetics
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Light*
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Membrane Proteins / chemistry
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Membrane Proteins / metabolism
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Oxygen / chemistry*
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Oxygen / metabolism
Substances
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Bacterial Proteins
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Membrane Proteins
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nickel-iron hydrogenase
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Hydrogenase
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Oxygen