Transient gene expression in Medicago truncatula leaves via Agroinfiltration

Kelsey Picard; Robyn Lee; Roger Hellens; Richard Macknight

doi:10.1007/978-1-62703-613-9_15

Transient gene expression in Medicago truncatula leaves via Agroinfiltration

Methods Mol Biol. 2013:1069:215-26. doi: 10.1007/978-1-62703-613-9_15.

Authors

Kelsey Picard¹, Robyn Lee, Roger Hellens, Richard Macknight

Affiliation

¹ Department of Biochemistry, University of Otago, Dunedin, New Zealand.

PMID: 23996318
DOI: 10.1007/978-1-62703-613-9_15

Abstract

Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in Medicago truncatula leaves using Agrobacterium tumefaciens infiltration. Using quantitative real-time PCR we demonstrate that the infiltration of a construct containing the LEGUME ANTHOCYANIN PRODUCTION 1 (LAP1) transcription factor results in the strong upregulation of key biosynthetic genes and the accumulation of anthocyanin pigment in the leaves after just 3 days. Thus, this method provides a rapid and powerful way to the discovery of downstream targets of M. truncatula transcription factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Plant
Gene Expression*
Medicago truncatula / genetics*
Medicago truncatula / growth & development
Medicago truncatula / microbiology*
Plant Leaves / genetics*
Plant Leaves / microbiology*
Plants, Genetically Modified
Transformation, Genetic*