Comparison of in vitro digestion characteristics and antioxidant activity of hot- and cold-pressed peanut meals

Food Chem. 2013 Dec 15;141(4):4246-52. doi: 10.1016/j.foodchem.2013.06.081. Epub 2013 Jun 28.

Abstract

Due to the poor protein solubility, hot-pressed peanut meal (HPM) has less value than cold-pressed peanut meal (CPM) in the food industry. The objective of this study was to determine whether the denatured proteins in HPM were suitable for hydrolysis by digestive enzymes. The hydrolysis characteristics and antioxidant activity of HPM and CPM during in vitro digestion were compared. The results showed that HPM was hydrolysed more extensively than CPM. There were more free amino acids and small peptides with MW<5 kDa in HPM hydrolysates. In addition, HPM hydrolysates displayed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,20-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical-scavenging activities, ORAC value and reducing power than CPM hydrolysates, which could be due to the higher content of small peptides, antioxidative amino acids and melanoidins in HPM hydrolysates. The above results indicated that HPM was a potential source of protein supplement for human consumption.

Keywords: Amino acid composition; Antioxidant activity; In vitro digestion; Molecular weight; Peanut meal.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / analysis
  • Antioxidants / analysis
  • Antioxidants / metabolism*
  • Arachis / chemistry
  • Arachis / metabolism*
  • Cold Temperature
  • Digestion*
  • Food Handling / methods*
  • Hot Temperature
  • Humans
  • Hydrolysis
  • Models, Biological
  • Peptides
  • Protein Hydrolysates / chemistry
  • Protein Hydrolysates / metabolism

Substances

  • Amino Acids
  • Antioxidants
  • Peptides
  • Protein Hydrolysates