Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7

Elena N Sigida; Yuliya P Fedonenko; Alexander S Shashkov; Evelina L Zdorovenko; Svetlana A Konnova; Vladimir V Ignatov; Yuriy A Knirel

doi:10.1016/j.carres.2013.07.013

Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7

Carbohydr Res. 2013 Oct 18:380:76-80. doi: 10.1016/j.carres.2013.07.013. Epub 2013 Aug 2.

Authors

Elena N Sigida¹, Yuliya P Fedonenko, Alexander S Shashkov, Evelina L Zdorovenko, Svetlana A Konnova, Vladimir V Ignatov, Yuriy A Knirel

Affiliation

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.

PMID: 23978662
DOI: 10.1016/j.carres.2013.07.013

Abstract

Lipopolysaccharide was obtained by phenol-water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (∼40%) 2-O-methylation of l-rhamnose.

Keywords: Azospirillum brasilense; Bacterial polysaccharide structure; Lipopolysaccharide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azospirillum brasilense / chemistry*
Azospirillum brasilense / classification
Carbohydrate Sequence
Molecular Sequence Data
O Antigens / chemistry*
O Antigens / isolation & purification

Substances

O Antigens