The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum

J Microbiol Methods. 2013 Nov;95(2):145-8. doi: 10.1016/j.mimet.2013.08.006. Epub 2013 Aug 23.

Abstract

A transformation method yielding up to 10(4) transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58°C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination.

Keywords: Archaea; Homologous recombination; Novobiocin; Plasmid; Thermoplasma acidophilum; Transformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomes, Archaeal / genetics
  • Cloning, Molecular
  • Culture Media
  • DNA, Archaeal / genetics
  • DNA, Circular / genetics
  • Drug Resistance, Microbial
  • Gene Transfer Techniques*
  • Genetic Vectors / genetics
  • Hydrogen-Ion Concentration
  • Novobiocin / pharmacology
  • Promoter Regions, Genetic
  • Sequence Analysis, DNA
  • Temperature
  • Thermoplasma / genetics*
  • Transformation, Genetic*

Substances

  • Culture Media
  • DNA, Archaeal
  • DNA, Circular
  • Novobiocin