Abstract
Inflammatory conditions mediated by activated microglia lead to chronic neuro-degenerative diseases such as Alzheimer's, Parkinson's, and Huntington's diseases. This study was conducted to determine the effect of floridoside isolated from marine red algae Laurencia undulata on LPS (100 ng/ml) activated inflammatory responses in BV-2 microglia cells. The results show that floridoside has the ability to suppress pro-inflammatory responses in microglia by markedly inhibiting the production of nitric oxide (NO) and reactive oxygen species (ROS). Moreover, floridoside down-regulated the protein and gene expression levels of iNOS and COX-2 by significantly blocking the phosphorylation of p38 and ERK in BV-2 cells. Collectively, these results indicate that floridoside has the potential to be developed as an active agent for the treatment of neuro-inflammation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cell Survival / drug effects
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Cyclooxygenase 2 / genetics
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Cyclooxygenase 2 / metabolism
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Down-Regulation
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Glycerol / analogs & derivatives*
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Glycerol / pharmacology
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Laurencia / chemistry
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Lipopolysaccharides / toxicity
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Mice
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Microglia / cytology
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Microglia / drug effects
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Microglia / metabolism
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Mitogen-Activated Protein Kinases / antagonists & inhibitors
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Mitogen-Activated Protein Kinases / metabolism*
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / genetics
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Nitric Oxide Synthase Type II / metabolism
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Phosphorylation
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Reactive Oxygen Species / metabolism
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Signal Transduction / drug effects*
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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2-galactopyranosylglycerol
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Lipopolysaccharides
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Reactive Oxygen Species
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Nitric Oxide
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NOS2 protein, human
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2
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Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases
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Glycerol