Abstract
The glycosylation of five different flavonols, fisetin, quercetin, myricetin, kaempferol, and 3-hydroxyflavone, was achieved by applying YjiC. 3-Hydroxyflavone was selected as a probe for in vitro glycorandomization of all flavonols using diverse nucleotide diphosphate-d/l-sugars. This study unlocked the possibilities of the glycodiversification of flavonols and the generation of novel compounds as future therapeutics.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus / enzymology
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Chromatography, High Pressure Liquid
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Drug Discovery / methods*
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Escherichia coli
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Flavonoids / chemistry
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Flavonoids / metabolism*
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Glycosylation
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Glycosyltransferases / metabolism*
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Metabolic Engineering / methods*
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Molecular Structure
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Nucleoside Diphosphate Sugars / metabolism
Substances
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Flavonoids
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Nucleoside Diphosphate Sugars
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Glycosyltransferases
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3-hydroxyflavone