Abstract
Four new ligustilides chuanxiongnolide R1 (1), chuanxiongnolide R2 (2), chuanxiongdiolide R1 (3) and chuanxiongdiolide R2 (4) together with eight known derivatives (5-12) were isolated from the root of Ligusticum chuanxiong Hort. Their structures were elucidated by HR-ESI-MS, UV, IR, 1D and 2D NMR (HSQC, HMBC, (1)H-(1)H COSY, NOESY) methods. The absolute configurations were confirmed via the circular dichroism (CD) spectrum. The anti-inflammatory assay in LPS-triggered RAW 264.7 macrophages was carried out on the twelve compounds. 1, 3, 5 and 6 showed significant inhibitory effects against LPS-induced NO production.
Keywords:
Anti-inflammatory activity; CD; Ligusticum chuanxiong Hort; Ligustilide.
© 2013.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Butyrolactone / analogs & derivatives*
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4-Butyrolactone / chemistry
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4-Butyrolactone / isolation & purification
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4-Butyrolactone / pharmacology
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4-Butyrolactone / therapeutic use
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Animals
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology
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Anti-Inflammatory Agents / therapeutic use*
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Benzofurans / chemistry
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Benzofurans / isolation & purification
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Benzofurans / pharmacology
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Benzofurans / therapeutic use*
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / pharmacology
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Drugs, Chinese Herbal / therapeutic use
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Inflammation / chemically induced
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Inflammation / drug therapy*
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Inflammation / metabolism
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Ligusticum / chemistry*
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Lipopolysaccharides
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Macrophages / drug effects
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Mice
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Molecular Structure
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Nitric Oxide / biosynthesis
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Phytotherapy*
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Plant Roots
Substances
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Anti-Inflammatory Agents
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Benzofurans
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Drugs, Chinese Herbal
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Lipopolysaccharides
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chuanxiongdiolide R1
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chuanxiongnolide R1
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Nitric Oxide
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ligustilide
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4-Butyrolactone