FITC-quencher based caspase 3-activatable nanoprobes for effectively sensing caspase 3 in vitro and in cells

Anming Tang; Bin Mei; Weijuan Wang; Wanglai Hu; Fang Li; Jun Zhou; Qing Yang; Hua Cui; Mian Wu; Gaolin Liang

doi:10.1039/c3nr03339b

FITC-quencher based caspase 3-activatable nanoprobes for effectively sensing caspase 3 in vitro and in cells

Nanoscale. 2013 Oct 7;5(19):8963-7. doi: 10.1039/c3nr03339b. Epub 2013 Aug 22.

Authors

Anming Tang¹, Bin Mei, Weijuan Wang, Wanglai Hu, Fang Li, Jun Zhou, Qing Yang, Hua Cui, Mian Wu, Gaolin Liang

Affiliation

¹ CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China. gliang@ustc.edu.cn.

PMID: 23970215
DOI: 10.1039/c3nr03339b

Abstract

By employing fluorescence resonance energy transfer (FRET) quenching, we rationally designed two new FITC-quencher based nanoprobes for effectively sensing caspase 3 (Casp3) in vitro and in cells. Our nanoprobes hold promise for assessing the chemotherapeutic effect of cancer treatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Apoptosis / drug effects
Apoptosis / radiation effects
Caspase 3 / analysis*
Caspase 3 / metabolism
Fluorescein-5-isothiocyanate / chemistry*
Fluorescence Resonance Energy Transfer*
Gold / chemistry
Hep G2 Cells
Humans
Metal Nanoparticles / chemistry
Microscopy, Confocal
Nanostructures / chemistry*
Peptides / chemistry
Peptides / metabolism
Ultraviolet Rays

Substances

Peptides
Gold
Caspase 3
Fluorescein-5-isothiocyanate