Abstract
Eleven steroidal saponins, dioscoreanosides A-K, along with five known congeners, were isolated from the flowers of Dioscorea bulbifera var. sativa. Their structures were established by extensive NMR experiments in conjunction with mass spectrometry. The isolated compounds were tested for cytotoxicity against urinary bladder carcinoma cells (ECV-304 cells). Our results revealed a moderate activity for spiroconazol A (15), pennogenin 3-O-α-l-rhamnopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→4)-[α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyranoside (12), and 26-O-ß-d-glucopyranosyl-(25R)-5-en-furost-3ß,17α,22α,26-tetraol-3-O-α-l-rhamnopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→4)-[α-l-rhamnopyranosyl-(1→2)]-β-d-glucopyranoside (13).
Keywords:
Cytotoxicity; Dioscorea bulbifera var. sativa; Dioscoreaceae; Pennogenin; Steroidal saponins.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology
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Antineoplastic Agents, Phytogenic / therapeutic use
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Dioscorea / chemistry*
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Flowers / chemistry*
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Humans
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Molecular Structure
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Phytosterols / chemistry
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Phytosterols / isolation & purification*
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Phytosterols / pharmacology
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Phytosterols / therapeutic use
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Phytotherapy
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use
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Saponins / chemistry
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Saponins / isolation & purification*
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Saponins / pharmacology
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Saponins / therapeutic use
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Urinary Bladder Neoplasms / drug therapy
Substances
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26-O-glucopyranosyl-5-en-furost-3,17,22,26-tetraol-3-O-rhamnopyranosyl-(1-4)-rhamnopyranosyl-(1-4)-(rhamnopyranosyl-(1-2))glucopyranoside
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Antineoplastic Agents, Phytogenic
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Phytosterols
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Plant Extracts
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Saponins
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pennogenin 3-O-rhamnopyranosyl-(1-4)-rhamnopyranosyl-(1-4)-(rhamnopyranosyl-(1-2))glucopyranoside
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spiroconazol A