Design, synthesis and biological studies of survivin dimerization modulators that prolong mitotic cycle

Bioorg Med Chem Lett. 2013 Oct 1;23(19):5429-33. doi: 10.1016/j.bmcl.2013.07.034. Epub 2013 Jul 24.

Abstract

Survivin, a member of the inhibitor of apoptosis protein (IAP) family proteins, has essential roles in cell division and inhibition of apoptosis. Several clinical studies in cancer patients have shown that the elevated levels of survivin correlate with aggressiveness of the disease and resistance to radiation and chemotherapeutic treatments. Survivin is an integral component of chromosomal passenger complex (CPC) where it binds to borealin and INCENP through its dimerization interface. Thus, disruption of functional survivin along its dimer interface with a small molecule is hypothesized to inhibit the proliferation of cancer cells and sensitize them to therapeutic agents and radiation. Recently, a small molecule (Abbott8) was reported to bind at the dimerization interface of survivin. Further development of this compound was accomplished by computational modeling of the molecular interactions along the dimerization interface, which has led to the design of promising survivin dimerization modulators. Two of the most potent survivin modulators, LLP3 and LLP9 at concentrations between 50 and 100nM, caused delay in mitotic progression and major mitotic defects in proliferating human umbilical vein endothelial cells (HUVEC) and prostate cancer cells (PC3).

Keywords: LLP3; LLP9; Survivin.

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Blotting, Western
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Chlorophenols / chemistry*
  • Chlorophenols / pharmacology*
  • Dimerization
  • Drug Design*
  • Humans
  • Inhibitor of Apoptosis Proteins / chemistry
  • Inhibitor of Apoptosis Proteins / metabolism*
  • Mitosis / drug effects*
  • Models, Molecular
  • Pyridones / chemistry*
  • Pyridones / pharmacology*
  • Survivin
  • Time Factors

Substances

  • 4-(3,5-bis(benzyloxy)phenyl)-6-(5-chloro-2-hydroxyphenyl)-2-oxo-1,2-dihydropyridine-3-carbonitrile
  • 6-(5-chloro-2-hydroxyphenyl)-2-oxo-4-phenyl-1,2-dihydropyridine-3-carbonitrile
  • Antineoplastic Agents
  • BIRC5 protein, human
  • Chlorophenols
  • Inhibitor of Apoptosis Proteins
  • Pyridones
  • Survivin