Surface modification of biomaterials for conjugation with human fetal osteoblasts

Chimia (Aarau). 2013;67(4):213-7. doi: 10.2533/chimia.2013.213.

Abstract

Surface functionalization of hydroxyapatite (HA) and beta-tricalcium phosphate (TCP) bioceramics with chemical ligands containing a pyrrogallol moiety was developed to improve the adhesion of bone cell precursors to the biomaterials. Fast and biocompatible copper-free click reaction with azido-modified human fetal osteoblasts resulted in improved cell binding to both HA and TCP bioceramics, opening the way for using this methodology in the preparation of cell-engineered bone implants.

MeSH terms

  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / metabolism
  • Calcium Phosphates / chemistry*
  • Calcium Phosphates / metabolism
  • Cell Adhesion*
  • Cell Proliferation
  • Cells, Cultured
  • Ceramics / chemistry*
  • Ceramics / metabolism
  • Click Chemistry
  • Durapatite / chemistry*
  • Durapatite / metabolism
  • Fetus / cytology
  • Fetus / metabolism*
  • Humans
  • Materials Testing
  • Molecular Structure
  • Osteoblasts / cytology
  • Osteoblasts / metabolism*
  • Surface Properties

Substances

  • Biocompatible Materials
  • Calcium Phosphates
  • beta-tricalcium phosphate
  • Durapatite