A convenient method to screen for carbapenemase-producing Pseudomonas aeruginosa

Damien Fournier; Pauline Garnier; Katy Jeannot; Amélie Mille; Anne-Sophie Gomez; Patrick Plésiat

doi:10.1128/JCM.01299-13

A convenient method to screen for carbapenemase-producing Pseudomonas aeruginosa

J Clin Microbiol. 2013 Nov;51(11):3846-8. doi: 10.1128/JCM.01299-13. Epub 2013 Aug 21.

Authors

Damien Fournier¹, Pauline Garnier, Katy Jeannot, Amélie Mille, Anne-Sophie Gomez, Patrick Plésiat

Affiliation

¹ Centre National de Référence de la Résistance aux Antibiotiques, Centre Hospitalier Régional Universitaire de Besançon, Besançon, France.

Abstract

A combined-disk method using imipenem and cloxacillin was evaluated to discriminate between carbapenemase-producing (n = 56) and nonproducing (n = 62) strains of Pseudomonas aeruginosa. With a cloxacillin load of 4,000 μg/disk, this very simple method showed a sensitivity and a specificity of 100%, irrespective of the type of carbapenemase produced.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / analysis*
Bacteriological Techniques / methods*
Cloxacillin / pharmacology
Humans
Pseudomonas Infections / microbiology*
Pseudomonas aeruginosa / enzymology*
Sensitivity and Specificity
beta-Lactamases / analysis*

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactamases
carbapenemase
Cloxacillin