Abstract
Testing of whole blood in miniaturized PCR is compromised by the opaque nature of whole blood that leads to physical masking of a fluorescent signal. We demonstrate a method to perform real-time PCR with whole blood that avoids interference from the opacity of whole blood.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Artifacts
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Blood / metabolism*
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Blood Chemical Analysis
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Gels
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Humans
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Molecular Diagnostic Techniques / methods*
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Point-of-Care Systems*
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Polymerase Chain Reaction / methods*
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Reagent Strips / chemistry*
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Real-Time Polymerase Chain Reaction