Alginate lyase and ciprofloxacin co-immobilization on biopolymeric microspheres for cystic fibrosis treatment

Macromol Biosci. 2013 Sep;13(9):1238-48. doi: 10.1002/mabi.201300134. Epub 2013 Aug 21.

Abstract

A new formulation is described based on biopolymeric microspheres containing alginate lyase (AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) and high-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix. ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solvents mixtures, obtaining Cip encapsulations from 46.0 to 100.0%. ALG-HMP shows a Cip sustainable release profile and is able to encapsulate 90.0% of AL, showing 76.0% enzyme activity after release under simulated intestinal conditions. The developed system is a promising delivery carrier to treat chronic infection of Pseudomonas aeruginosa and to reduce the viscoelasticity of the mucus accumulated into intestine of CF patients.

Keywords: alginate lyase; biopolymers; ciprofloxacin; cystic fibrosis; drug delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopolymers / chemistry*
  • Ciprofloxacin / pharmacology
  • Ciprofloxacin / therapeutic use*
  • Cystic Fibrosis / drug therapy*
  • Enzymes, Immobilized / metabolism*
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • Microscopy, Electron, Scanning
  • Microspheres*
  • Pectins / chemistry
  • Polysaccharide-Lyases / metabolism*
  • Propylene Glycol / chemistry
  • Solvents

Substances

  • Biopolymers
  • Enzymes, Immobilized
  • Solvents
  • Ciprofloxacin
  • Propylene Glycol
  • Pectins
  • Polysaccharide-Lyases
  • poly(beta-D-mannuronate) lyase