Abstract
Trans-activation response DNA-binding protein of 43 kDa (TDP-43), a key factor in several neurodegenerative conditions, was discovered as a novel target of heteronemin by chemical proteomics. Combining bio-physical orthogonal approaches with biological analysis, heteronemin was found to influence the binding of TDP-43-cognate nucleic acids and to modulate the TDP-43 aggregation state and its cellular localization.
MeSH terms
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Animals
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DNA, Single-Stranded / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism*
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HeLa Cells
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Humans
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Microscopy, Confocal
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NF-kappa B / metabolism
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Neurodegenerative Diseases / metabolism
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Neurodegenerative Diseases / pathology
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Porifera / chemistry*
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Porifera / metabolism
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Protein Binding
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Terpenes / chemistry
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Terpenes / isolation & purification
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Terpenes / metabolism*
Substances
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DNA, Single-Stranded
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DNA-Binding Proteins
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NF-kappa B
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Terpenes
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heteronemin