Cyclodextrin-based microcapsules as bioreactors for ATP biosynthesis

Biomacromolecules. 2013 Sep 9;14(9):2984-8. doi: 10.1021/bm400584h. Epub 2013 Aug 26.

Abstract

A biomimetic energy converter was fabricated via the assembly of CF0F1-ATPase on lipid-coated hollow nanocapsules composed of α-cyclodextrins/chitosan-graft-poly(ethylene glycol) methacrylate. Upon entrapped GOD into these capsules, the addition of glucose could trigger proton-motive force and then drive the rotation of ATPase to synthesize ATP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Biocatalysis
  • Bioreactors
  • Chitosan / analogs & derivatives
  • Chitosan / chemistry
  • Cyclodextrins / chemistry*
  • Glucose / chemistry
  • Glucose Oxidase / chemistry
  • Hydrogen-Ion Concentration
  • Kinetics
  • Lipids / chemistry
  • Mitochondrial Proton-Translocating ATPases / chemistry*
  • Nanocapsules / chemistry*
  • Particle Size
  • Polyethylene Glycols / chemistry
  • Polymethacrylic Acids / chemistry

Substances

  • Cyclodextrins
  • Lipids
  • Nanocapsules
  • PEG-graft-trimethyl-chitosan copolymer
  • Polymethacrylic Acids
  • Polyethylene Glycols
  • Adenosine Triphosphate
  • Chitosan
  • Glucose Oxidase
  • mitochondrial ATPase subunit c
  • Mitochondrial Proton-Translocating ATPases
  • Glucose