The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting

Shen Y Heazlewood; Brenda Williams; Melonie J Storan; Susan K Nilsson

doi:10.1007/978-1-62703-508-8_10

The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting

Methods Mol Biol. 2013:1035:121-33. doi: 10.1007/978-1-62703-508-8_10.

Authors

Shen Y Heazlewood¹, Brenda Williams, Melonie J Storan, Susan K Nilsson

Affiliation

¹ Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organization, Melbourne, VIC, Australia.

PMID: 23959986
DOI: 10.1007/978-1-62703-508-8_10

Abstract

Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

MeSH terms

Animals
Bone Marrow Cells / physiology*
Cell Survival
Cells, Cultured
Flow Cytometry
Immunomagnetic Separation
Megakaryocytes / physiology*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Platelet Membrane Glycoprotein IIb / metabolism
Polyploidy

Substances

Platelet Membrane Glycoprotein IIb