Abstract
Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / genetics
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Antibodies / immunology*
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Antibody Affinity*
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Binding Sites, Antibody
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Cell Line
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Escherichia coli / genetics
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Histones / chemistry
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Histones / genetics
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Histones / immunology*
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Humans
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Lysine / chemistry
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Lysine / genetics
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Lysine / immunology*
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Peptide Library
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Protein Processing, Post-Translational*
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Sensitivity and Specificity
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Single-Chain Antibodies / genetics
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Single-Chain Antibodies / immunology
Substances
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Antibodies
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Histones
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Peptide Library
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Single-Chain Antibodies
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Lysine