Abstract
Background:
We sought to assess the expression of five cell stress molecules: Heat shock protein (HSP)-60, HSP-70, interferon (IFN)-α, rat retinoic acid early induible-1 like transcript (RRLT), and vasoactive intestinal peptide (VIP) during rat chronic small bowel allograft rejection.
Methods:
A rat model of small bowel allograft rejection was induced on postoperative day 90 using F344 rat as donor and Lewis rat as recipient.
Results:
We demonstrated increased expression of lamina propria HSP-60 (mean rank grade 8.3 and 3.2, respectively), graft RRLT (relative mRNA amount 5.1 ± 1.8 and 3.0 ± 1.3, respectively) and IFN-α (relative mRNA amount 108.3 ± 49.2 and 59.2 ± 22.0, respectively) in allograft group compared with the isograft group.
Conclusion:
These data suggest the presence of cell stress during chronic small bowel allograft rejection.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Allografts
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Animals
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Chaperonin 60 / genetics
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Chaperonin 60 / metabolism
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Chronic Disease
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Disease Models, Animal
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Gene Expression Regulation
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Graft Rejection / etiology*
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Graft Rejection / genetics
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Graft Rejection / metabolism
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Graft Rejection / pathology
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HSP70 Heat-Shock Proteins / genetics
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HSP70 Heat-Shock Proteins / metabolism
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Interferon-alpha / genetics
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Interferon-alpha / metabolism
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Intestine, Small / metabolism
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Intestine, Small / pathology
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Intestine, Small / transplantation*
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Male
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / metabolism
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RNA Helicases / genetics
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RNA Helicases / metabolism
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RNA, Messenger / metabolism
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Rats
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Rats, Inbred F344
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Rats, Inbred Lew
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Stress, Physiological*
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Time Factors
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Vasoactive Intestinal Peptide / genetics
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Vasoactive Intestinal Peptide / metabolism
Substances
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Chaperonin 60
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HSP70 Heat-Shock Proteins
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Hspd1 protein, rat
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Interferon-alpha
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Mitochondrial Proteins
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RNA, Messenger
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Vasoactive Intestinal Peptide
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RIG-I protein, rat
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RNA Helicases