The epigenetically-regulated miR-663 targets H-ras in K-562 cells

FEBS J. 2013 Oct;280(20):5109-17. doi: 10.1111/febs.12485. Epub 2013 Sep 12.

Abstract

miR-663 is a tumour suppressor that is potentially regulated by modification of CpG islands. Whether aberrant methylation is one of the reasons for miR-663 down-regulation in some malignant cells and whether miR-663 targets oncogenes warrants further research. In the present study, we report that the CpG islands in the upstream region of pre-miR-663 are aberrantly methylated in the k-562 cell line and in the white blood cells of some chronic myelogenous leukaemia patients, and also that H-ras is one of the genes targeted by miR-663. Over-expression of miR-663 may suppress proliferation of the k-562 cell line in part by enhancing cell apoptosis.

Keywords: DNA methylation; H-ras; MiR-663; epigenetics; gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Azacitidine / pharmacology
  • Base Sequence
  • Cell Cycle
  • Cell Proliferation
  • CpG Islands
  • DNA Methylation / drug effects
  • DNA Primers
  • Epigenesis, Genetic*
  • Genes, Tumor Suppressor
  • Genes, ras*
  • Humans
  • K562 Cells
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Polymerase Chain Reaction

Substances

  • DNA Primers
  • MIRN663 microRNA, human
  • MicroRNAs
  • Azacitidine