Abstract
Bioorthogonal covalent cross-linking of DNA-binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS-modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine-containing peptides and proteins by Michael addition.
Keywords:
DNA; DNA polymerase; Michael additions; bioorthogonal chemistry; proteins.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrylamide / chemical synthesis
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Acrylamide / chemistry*
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Cross-Linking Reagents / chemistry
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DNA / chemical synthesis
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DNA / chemistry*
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DNA-Directed DNA Polymerase / chemistry
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Ethylenes / chemistry
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Models, Molecular
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Proteins / chemistry*
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Proteins / metabolism
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Sulfonamides / chemical synthesis
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Sulfonamides / chemistry*
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Sulfonic Acids / chemistry
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Vinyl Compounds / chemical synthesis
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Vinyl Compounds / chemistry*
Substances
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Cross-Linking Reagents
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Ethylenes
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Proteins
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Sulfonamides
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Sulfonic Acids
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Vinyl Compounds
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Acrylamide
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DNA
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DNA-Directed DNA Polymerase
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ethylenesulfonic acid