Mapping functional interaction sites of human prune C-terminal domain by NMR spectroscopy in human cell lysates

Abstract

Get well prune: The C-terminal third domain of h-prune is largely unfolded and involved in relevant protein-protein interactions, particularly with Nm23-H1 (see figure), GSK-3β and gelsolin. This study shows that protein functions mediated by protein-protein interactions can be accurately followed in cell lysates by using fast NMR spectroscopy, which could be easily used for a very efficient NMR drug-discovery strategy.

Keywords: NMR spectroscopy; human cell lysates; intrinsically disordered proteins; protein-protein interactions; structure elucidation.