Involvement of anthocyanins in the resistance to chilling-induced oxidative stress in Saccharum officinarum L. leaves

Abstract

Whether anthocyanins elevate resistance to chilling-induced oxidative stress in Saccharum officinarum L. cv Badila seedlings is investigated. Plants with four fully expanded leaves were exposed to chilling stress (8 °C/4 °C, 11 h photoperiod) for 3 days and then transferred to rewarming condition (25 °C/20 °C, 11 h photoperiod) for another 2 days. At the end of the chilling period, H2O2 and superoxide radical (O2-) levels increased sharply and were near the same in the central (CL) and the final fully expanded leaves (FL). Moreover, the degree of chilling injury indicated by malonaldehyde concentration and percent of ion leakage also was near the same. Most of the tested parameters returned near to the control level after 2 days of rewarming. With further analyzing, we found that superoxide dismutase (EC 1.15.1.1), ascorbate peroxidase (EC 1.11.1.11), glutathione reductase (EC 1.6.4.2) activities increased much higher and catalase (EC 1.11.1.6) activity and ascorbate/dehydroascorbate ratio decreased much more in FL than CL in response to chilling. However, anthocyanins concentration coupling with glutathione/oxidized glutathione increased much higher in CL than FL under chilling stress. These finds suggest that anthocyanins at least partially compensate the relative deficiency of antioxidants in CL compared with FL. α,α-Diphenyl-β-picrylhydrazyl assays further confirmed this idea. The relationships between anthocyanins and antioxidants were analyzed and the possible mechanisms of the affection of anthocyanins on antioxidant metabolism were discussed.

Keywords: APX; ASC; Anthocyanin; Antioxidant; CAT; CL; Chewing cane; Chilling; DHAR; DPPH; FL; GR; GSH; GSSG; H(2)O(2); MDA; O(2)(−); PPF; ROS; Reactive oxygen species; SOD; ascorbate; ascorbate peroxidase; catalase; central leaves; dehydroascorbate reductase; final fully expanded leaves; glutathione reductase; hydrogen peroxide; malondialdehyde; oxidized glutathione; photosynthetic photon flux; reactive oxygen species; reduced glutathione; superoxide dismutase; superoxide radical; α,α-diphenyl-β-picrylhydrazyl.