Abstract
Here we report a case of co-infection with Orientia tsutsugamushi, the causative agent of scrub typhus, and Arsenophonus nasoniae in a woman with a rash and an eschar who returned from a trip to Southeast Asia. A. nasoniae was previously considered to be a secondary insect and tick endosymbiont of unknown pathogenicity in humans. We amplified both O. tsutsugamushi and A. nasoniae DNA from a skin eschar with qPCR, and a seroconversion for O. tsutsugamushi and A. nasoniae was observed with immunofluorescence assays and western blotting for this patient. And we used 2-D western blotting with an A. nasoniae antigen and polyclonal mouse anti-A. nasoniae antibodies produced in our laboratory to detect the specific antigenic A. nasoniae proteins.
MeSH terms
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Adult
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Animals
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Antibodies, Bacterial / blood*
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Antibodies, Bacterial / immunology
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Antigens, Bacterial / immunology
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Antigens, Bacterial / isolation & purification
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Asia, Southeastern
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism
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Biopsy
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Coinfection
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Enterobacteriaceae / genetics
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Enterobacteriaceae / immunology
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Enterobacteriaceae / isolation & purification*
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Enterobacteriaceae Infections / complications*
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Enterobacteriaceae Infections / diagnosis
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Enterobacteriaceae Infections / microbiology
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Female
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France
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Humans
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Mice
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Mice, Inbred BALB C
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Orientia tsutsugamushi / genetics
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Orientia tsutsugamushi / immunology
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Orientia tsutsugamushi / isolation & purification*
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Polymerase Chain Reaction
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Scrub Typhus / complications*
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Scrub Typhus / diagnosis
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Scrub Typhus / microbiology
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Skin / microbiology*
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Skin / pathology
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Travel
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Proteins