Histometric analyses of cancellous and cortical interface in autogenous bone grafting

Int J Clin Exp Pathol. 2013 Jul 15;6(8):1532-7. Print 2013.

Abstract

Surgical procedures involving the rehabilitation of the maxillofacial region frequently require bone grafts; the aim of this research was to evaluate the interface between recipient and graft with cortical or cancellous contact. 6 adult beagle dogs with 15 kg weight were included in the study. Under general anesthesia, an 8 mm diameter block was obtained from parietal bone of each animal and was put on the frontal bone with a 12 mm 1.5 screws. Was used the lag screw technique from better contact between the recipient and graft. 3-week and 6-week euthanized period were chosen for histometric evaluation. Hematoxylin-eosin was used in a histologic routine technique and histomorphometry was realized with IMAGEJ software. T test was used for data analyses with p<0.05 for statistical significance. The result show some differences in descriptive histology but non statistical differences in the interface between cortical or cancellous bone at 3 or 6 week; as natural, after 6 week of surgery, bone integration was better and statistically superior to 3-week analyses. We conclude that integration of cortical or cancellous bone can be usefully without differences.

Keywords: Bone defects; animal study; bone grafting.

MeSH terms

  • Animals
  • Bone Transplantation / methods*
  • Dogs
  • Frontal Bone / transplantation*
  • Parietal Bone / transplantation*
  • Transplantation, Autologous