Quantitative analysis of initial adhesion of bacterial vaginosis-associated anaerobes to ME-180 cells

António Machado; Débora Salgueiro; Michael Harwich; Kimberly Kay Jefferson; Nuno Cerca

doi:10.1016/j.anaerobe.2013.07.007

Quantitative analysis of initial adhesion of bacterial vaginosis-associated anaerobes to ME-180 cells

Anaerobe. 2013 Oct:23:1-4. doi: 10.1016/j.anaerobe.2013.07.007. Epub 2013 Aug 2.

Authors

António Machado¹, Débora Salgueiro, Michael Harwich, Kimberly Kay Jefferson, Nuno Cerca

Affiliation

¹ IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298-0678, USA. Electronic address: machadobq@deb.uminho.pt.

PMID: 23916636
DOI: 10.1016/j.anaerobe.2013.07.007

Abstract

Bacterial vaginosis is the leading vaginal disorder but the transition from health to this dysbiotic condition remains poorly characterized. Our goal was to quantify the ability of BV-associated anaerobes to adhere to epithelial cells in the presence of lactobacilli. Gardnerella vaginalis outcompeted Lactobacillus crispatus and Lactobacillus iners actually enhanced its adherence.

Keywords: Bacterial vaginosis; Gardnerella vaginalis; Lactobacilli; ME-180; Vaginal epithelial cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria, Anaerobic / physiology*
Bacterial Adhesion*
Epithelial Cells / microbiology*
Female
Gardnerella vaginalis / physiology*
Humans
Lactobacillus / physiology*
Vaginosis, Bacterial / microbiology*